Fluorescence-Amplifying Assay for Irradiated DNA Lesions Using Water-Soluble Conjugated Polymers

产品简述

期刊

Macromolecular Rapid Communications


标题

Fluorescence-Amplifying Assay for Irradiated DNA Lesions Using Water-Soluble Conjugated Polymers


作者

Fude Feng,Xinrui Duan,Shu Wang


摘要

A new platform has been developed for DNA lesion detection using a cationic conjugated polymer (CCP). DNA that contains two adjacent thymine bases is irradiated with ultraviolet light to allow for the formation of cyclobutane pyrimidine dimers and pyrimidine–pyrimidone dimers. The DNA lesions block the primer extension, and the base labeled with fluorescein cannot be incorporated into the DNA strand. Addition of the CCP leads to inefficient fluorescence resonance energy transfer (FRET) from CCP to fluorescein. For the case without DNA lesions, successful primer extension allows for efficient FRET between them. In view of the FRET signal changes, the DNA lesions can be detected. This new protocol offers a convenient detection for DNA lesions in aqueous solution without any isolation and washing steps.


原文链接

http://onlinelibrary.wiley.com/doi/10.1002/marc.200800543/abstract



2017年01月06日

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